HPLC Column, RP C4 Butyl, Wide Pore, 10um, 4.0mm ID x 50mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. Classified as USP L26, this stationary phase provides moderate hydrophobic retention while offering enhanced accessibility for larger molecules through its 300 Å pore structure. The C4 chemistry is widely used for proteins, peptides, enzymes, and other large biomolecules where reduced hydrophobic retention and improved mass transfer are advantageous compared to longer-chain alkyl phases.
68004-05P-4-3M is a 50 mm x 4.0 mm ID analytical HPLC column packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The short 50 mm column length is optimized for rapid analyses, fast screening studies, and bioanalytical method development. The larger 10 µm particles support higher flow rates with lower backpressure, making the column particularly useful for biomolecule separations, preparative method scouting, and applications where operational robustness is important. The 4.0 mm ID format is commonly selected for legacy methods and conventional HPLC systems and typically operates at approximately 0.8–1.5 mL/min.
This USP L26 column is particularly useful for proteins, peptides, enzymes, and other large hydrophobic or neutral compounds analyzed by reversed-phase chromatography. The combination of fully endcapped C4 chemistry, high-purity silica, wide pore architecture, and larger particle size provides excellent recovery, mass transfer, and chromatographic performance for biomolecular and bioanalytical applications. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C4 Butyl Wide Pore stationary phase
USP L26 classification
Fully double endcapped chemistry
Traditional butyl (C4) bonded phase
300 Å wide pore structure for large molecules
High-purity silica support
10 µm particles for lower backpressure
Suitable for higher flow rates
Excellent for protein and peptide separations
Ideal for bioanalytical applications
Typical Applications
Reversed-phase HPLC
USP L26 methods
Protein analysis
Peptide analysis
Enzyme analysis
Biomolecule characterization
Bioanalytical methods
Preparative method scouting
Method development
Large molecule separations
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4 Butyl Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 50 mm
Length: 50 mm
Recommended Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
This item is a Special Order Item manufactured specifically to customer requirements.
Special Order Items cannot be canceled or returned once an order has been placed.
Manufactured upon receipt of order.
May have extended lead times compared to stocked products.
Important Notes
Designed for reversed-phase chromatography of large molecules
Wide pore structure improves accessibility for proteins and peptides
Provides less hydrophobic retention than comparable C8 columns
Larger particle size reduces operating backpressure
Excellent choice for biomolecule characterization and purification
Compatible with conventional HPLC systems
Alternative for many USP L26 methods
Special Order Item